Predict what you expect to see each of the two NA AMP plates shown in the Transformation Protocol in both visible light and UV light. You should have 4 predictions. We have provided a few examples of answers to help guide you.
E. coli + pGRN plasmid on nutrient agar (NA)
E. coli + pGRN plasmid on nutrient agar with ampicillin (AMP)
HINT: For the 3rd data row (+pGRN AMP, visible), you should predict the number of colonies relative to the 1000 predicted first data row. So - do you expect more than 1000, less than 1000 or equal to 1000.
pGRN added? |
Plate |
Light |
Approximate number of colonies and color |
+ |
NA |
visible |
Example: 1000 colonies, all white |
+ |
NA |
UV |
Example: 1000 colonies, only a few glow green |
+ |
AMP |
visible |
|
+ |
AMP |
UV |
Give an explanation for your two predictions – both numbers and color.
pGRN added? |
Plate |
Light |
Approximate number of colonies and color |
+ |
NA |
visible |
Example: 1000 colonies, all white |
+ |
NA |
UV |
Example: 1000 colonies, only a few glow green |
+ |
AMP |
visible |
The colonies are must less than 1000 (transformation rate is very low so that number may be double digit), COLOUR: WHITE COLOUR |
+ |
AMP |
UV |
Less than 1000 - colour green. |
pGRN plasmid has ampicillin resistance gene, the succ successful transformed bacteria can survive in the medium with AMPICILLIN. But the number of transformed cells are very low in number
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