Question

	Will bacteria grow?	Will bacteria glow under UV light?
Plate 1 (LB only) w/pGlo-	Prediction: yes Reason: because there is no antibiotic present the wild-type bacteria grew normally and formed a "lawn" across the whole plate.	Prediction: Reason:
Plate 2 (LB + amp) w/pGlo-	Prediction: no Reason: because the bacterial cell walls are unable to form and the bacterial cells die.	Prediction: Reason:
Plate 3 (LB + amp) w/pGlo+	Prediction: yes Reason: because the plasmid also contains the gene allowing for antibiotic resistance. These bacteria will also fluoresce green.	Prediction: Reason:
Plate 4 (LB + amp + ara) w/pGlo+	Prediction: yes Reason: Plasmid must confer resistance to ampicillin.	Prediction: yes Reason: Antibiotics usually kill bacteria (are bacteriocidal) or inhibit their growth

Answer 1

Plate 1:

Prediction: Bacteria will not glow under UV light. Reason: This is because the plasmid carrying the Glo gene has not been transformed.

Plate 2:

Prediction: No. Reason: there is no growth, no question of glowing bacteria.

Plate 3:

Prediction: No. Reason: the bacteria now carries the Glo gene specific plasmid, which will transcribe the gene and then translate it. However, the GFP gene which imparts fluorescence is expressed only in presence of arabinose.

Plate 4:

Prediction: Yes. Reason: the bacteria now carries the Glo gene specific plasmid, which will transcribe the gene and then translate it, which will allow it to respond to UV light.