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We are separating compounds on a column. What physical property do we use to distinguish these...

We are separating compounds on a column. What physical property do we use to
distinguish these compounds? Why does a hypothetical compound A elute faster than a
hypothetical compound B? (apply to normal and reverse phase)

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Answer #1

The mixture of compounds is separated by carrying it on a mobile phase through a stationary phase. The stationary phase of column chromatography is a glass column filled with alumina or silica gel whereas the mobile phase is a solvent, or a mixture of two solvents which is made to travel down the column, making it possible for different compounds to travel at different rates. This process is based on the principle of differential adsorption of substance by the adsorbent.

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