Question

1.A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is the _____ blot.
Select one:
a. Northern
b. Western
c. Northeastern
d. Southern
e. Southwestern

2. DNA sequencing by controlled termination of replication is called the _____ method.
Select one:
a. Sanger
b. E. coli
c. Restriction enzymes
d. DNA Microarray
e. Ligase
f. Polymerase Chain Reaction
g. Footprint
h. Reverse Trancriptase
i. Vector
j. Expression
k. Fluorescent
l. cDNA

3. How does the genome differ from the transcriptome?
Select one:
a. The genome does not contain untranslated regions of the DNA but the transcriptome include all transcribed regions of the DNA.
b. The genome is all of the genes in the DNA of a species, whereas the transcriptome is only those that are unique to an individual.
c. The genome is the genes that encode for proteins in a cell, whereas the transcriptome is only those proteins that are needed for steady-state levels of a particular cell.
d. The transcriptome is all genes that can be transcribed by a cell, whereas the genome is the complete DNA sequence of the cell.
e. The genome is all of the genes in the DNA of a cell, whereas the transcriptome is only those that are expression within a specific tissue.

4. In the controlled termination method of DNA sequencing, in what direction do you read the nucleotide sequence and why?
Select one:
a. Reading the gel from the top to the bottom gives the sequence in the 5? to 3? direction; shorter fragments that were terminated early in polymerization move faster down the gel.
b. Reading the gel from the bottom to the top gives the sequence in the 5? to 3? direction; longer fragments that were terminated later in polymerization move faster down the gel.
c. Reading the gel from the bottom to the top gives the sequence in the 5? to 3? direction; shorter fragments that were terminated early in polymerization move faster down the gel.
d. Reading the gel from the top to the bottom gives the sequence in the 5? to 3? direction; longer fragments that were terminated early in polymerization move faster down the gel.
e. Reading the gel from the bottom to the top gives the sequence in the 3? to 5? direction; shorter fragments that were terminated early in polymerization move faster down the gel.

5. Instead of radioactivity, current DNA sequencing commonly uses _____ base analogues.
Select one:
a. cDNA
b. E. coli
c. Reverse Trancriptase
d. Expression
e. Footprint
f. Polymerase Chain Reaction
g. Fluorescent
h. Ligase
i. Restriction enzymes
j. Sanger
k. DNA Microarray
l. Vector

6. Plasmids used in recombinant DNA technology typically:
Select one:
a. are circular double-stranded molecules.
b. possess a gene for antibiotic resistance and replicate independently of the host genome.
c. replicate independently of the host genome.
d. All of the answers are correct.
e. possess a gene for antibiotic resistance.

7. Reagents necessary for sequencing by chain termination include:
Select one:
a. template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase.
b. template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase.
c. All of the answers are correct.
d. template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe.
e. None of the answers is correct.

8. Reverse transcriptase is normally found in:
Select one:
a. plants.
b. All of the answers are correct.
c. None of the answers is correct.
d. retrovirus.
e. mitochondria.

9. The probe used to isolate a gene from a genomic library is often:
Select one:
a. its promoter region.
b. All of the answers are correct.
c. a portion of the mRNA of the gene.
d. the ligand that binds to the protein.
e. None of the answers is correct.

10. The specificity or stringency of a PCR reaction can be controlled by altering the reaction:
Select one:
a. All of the answers are correct.
b. target sequence.
c. temperature and salt concentration.
d. volume.
e. None of the answers is correct.

11. The type of vector used for synthesis of protein is _____.
Select one:
a. Fluorescent
b. Ligase
c. Restriction enzymes
d. Sanger
e. cDNA
f. DNA Microarray
g. Expression
h. Footprint
i. Reverse Trancriptase
j. E. coli
k. Vector
l. Polymerase Chain Reaction

12. What do Southern, Northern, and Western blots detect, respectively?
Select one:
a. DNA, protein, and RNA
b. RNA, protein, and DNA
c. RNA, DNA, and protein
d. DNA, RNA, and protein
e. protein, DNA, and RNA

13. Which of the following DNA sequences contains a 4–8 base palindromic site? (Note: Only one strand is shown.)
Select one:
a. GAGAGAGA
b. CGATTAGC
c. GCATCC
d. GCATATGC
e. CAGTCC

14. _____ A collection of DNA sequences representing all of the mRNA expressed in a particular cell type.
Select one:
a. Restriction enzymes
b. Vector
c. E. coli
d. cDNA
e. Fluorescent
f. DNA Microarray
g. Footprint
h. Reverse Trancriptase
i. Expression
j. Sanger
k. Ligase
l. Polymerase Chain Reaction

15. _____ are enzymes that split DNA at specific base-pair sequences.
Select one:
a. Reverse Trancriptase
b. Vector
c. Polymerase Chain Reaction
d. Footprint
e. cDNA
f. E. coli
g. Fluorescent
h. Ligase
i. Sanger
j. Expression
k. Restriction enzymes
l. DNA Microarray

Answer 1

Answer 1) a is the right answer.

Northern blotting is used to isolate the RNA.

Southern blotting is used to isolate the DNA

Western blotting is used to isolate the protein.

Answer 2) a is the answer.

Sanger sequencing method is used to identify the sequence of DNA by termination method. The deoxyribonucleotides, primers, DNA Polymerase, template and the dideoxyribonucleotides are added in the reaction. The dideoxyribonucleotides are useful in termination reactions. The newly synthesised strand is loaded on gel and then the sequence is identified. It's complementary sequence is of template DNA.