Question

complete the sentences with the appropriate term or phrase and arrange the sentences in order from what happen first to last.

Pour molten agarose into an acrylic plate to which a comb is attached.

Place the hardened gel into a tank with buffered solution and remove the comb.

Load DNA samples mixed with a blue dye into the wells.

Attach the positive and negative electrodes to a power supply and turn on the current.

Negatively charged DNA molecules will move toward the positive electrode, with small molecules traveling faster than large molecules.

Remove the gel from the tank and stain the DNA.

View the gel under UV light to locate the DNA and estimate size.

Answer 1

1. Pour molten agarose into an acrylic plate to which a comb is attached.

2. Place the hardened/solidified gel into a tank with a buffered solution and remove the comb.

3. Load DNA samples mixed with a blue dye into the wells.

4. Attach the positive and negative electrodes to a power supply and turn on the current.

5. Negatively charged DNA molecules will move toward the positive electrode, with small molecules traveling faster than large molecules.

6. Remove the gel from the tank and stain the DNA.

7. View the gel under UV light to locate the DNA and estimate the size.

Yes, you have done quite well. All the words/phrases used to fill the blanks are correct and steps or the workflow is also okay. Just remember, sometimes for agarose gel electrophoresis when EtBr is used for DNA staining, you need to add the EtBr in the molten agar and mix thoroughly before pouring into the gel caster.