Question

Consider the following scenarios and select which DNA technology is the best to use, options listed after scenarios.

A. You want to sequence several DNA fragments within a rare bird species' genome, but you don't know anything about the species' genome. Select one: Illumina sequencing, Sanger sequencing, Gene cloning OR PCR

B. You want to make copies of an STR locus in order to determine the genotype of a patient. Because this is a human STR, the sequence near the STR is well-known. Select one: Illumina sequencing, Sanger sequencing, Gene cloning or PCR

C. You seek to determine the nucleotide sequence of a single gene associated with flower color in roses, a species with a well-studied genome. You have a tight budget, so you need to do this in the most inexpensive way. Select one: Illumina sequencing, Sanger sequencing, Gene cloning or PCR

D. You want to make copies of a DNA fragment and it is especially important that there are minimal misincorporations in the replicated fragments' sequence. Select one: Illumina sequencing, Sanger sequencing, Gene cloning or PCR

E. You are comparing the genome of a patient with a rare autosomal recessive disease to the sequenced genome of a healthy individual in search for a SNP that could potentially be causing the disease phenotype. You seek to use a DNA technology that works rapidly to sequence the patient's entire genome so that you can quickly work towards identifying the disease-causing allele. Select one: Illumina sequencing, Sanger sequencing, Gene cloning or PCR

Answer 1

A. Illumina sequencing
It is used to sequences large unknown nucleic acid sequences
It is an expensive but rapid and reliable method.

B. PCR
It is an in vitro method for DNA amplification.
It is an inexpensive and rapid method

C. Sanger sequencing
It is used to sequence short stretches of nucleic acids.
Primer sequences must be known for this process.

D. Gene cloning
It is an In vivo method to amplify target gene sequences with high fidelity

E. Illumina sequencing
It is used to sequences large unknown nucleic acid sequences
It is also used for Genome wide analysis studies (GWAS)