Question

1. Which of the following is the correct definition of synteny

a) The conservation of gene order within two genomes

b) The conservation of gene function within two genomes

c) The percentage of nucleotide sequence identity between two genomes

d) The percentage of amino acid sequence identity between two genomes

2. Why is open reading frame scanning not applicable when searching for functional RNA genes?
a. the codons for functional RNA molecules are not well understood
b. introns make the identification of functional RNAs more difficult
c. functional RNA molecules are not composed of codons
d. the genetic code is not universal
e. the rules for specifying the start and stop regions of functional RNA genes are not well udnerstood

3. Intrastrand base pairing can interfere with which of the following methods in DNA sequencing?

a.      Chemical degradation method

b.      Pyrosequencing

c.      Chain termination method

d.      Clone Contig method

4. Chromosome walking is best described as:

a) aligning DNA sequences by computer, to generate contigs

b) Generating a map along a chromosome in a step by step manner

c) Identifying clones whose inserts overlap to generate a library of clones that cover a given segment of DNA

d) Sequencing a genome on one clone at a time to ensure that no gaps are present at the end of the project

5. Chromosome walking is best described as:

a) aligning DNA sequences by computer, to generate contigs

b) Generating a map along a chromosome in a step by step manner

c) Identifying clones whose inserts overlap to generate a library of clones that cover a given segment of DNA

d) Sequencing a genome on one clone at a time to ensure that no gaps are present at the end of the projectIntrastrand base pairing can interfere with which of the following methods in DNA sequencing

6. Which of the following is NOT true about Next Generation Sequencing

a) Next generation sequencing includes pyrosequencing and Illumina sequencing

b) Next generation sequencing sequences smaller fragmented DNA than Sanger sequencing

c) Next generation sequncing visualize the sequence by fluorescent

d) NExt generation sequencing does not require DNA polymerase

Answer 1