Question

Why is protein folding in-vitro and in-vivo considered to be different?

Answer 1

Protein folding is the process by which linear information contained in amino acid sequence of polypeptide chain gives rise to three dimensional conformation of functional protein.

In vivo folding was long considered as autonomous process unaffected by other proteins and cellular components.

Protein initially fold in vivo upon their biosynthesis .They are subjected to created by ribosome associated enzymes and chaperone.protein folding in vivo done at low dilution. In contrast folding of protein in vitro initiated from an unfolded ensemble in which population of full length chains is subjected to folding condition. protein folding in vitro done at high dilution.