Question

In electrophoresis, can multiple pure bands be indicative of a single protein with several domains? I know it can be indicative of different subunits of a complex.

Answer 1

In gel electrophoresis proteins can be separated in a mixture of proteins of different sizes or can separate the subunits of a tertiary protein.

Case 1)

If you run a single pure protein without denaturation then you will get a single band.

Or

Run different sizes of different proteins can get different bands based on their size.

Case 2).

If you want to know how many subunits of a tertiary protein then you can add denaturation substance Eg SDS, to the protein and run the gel then you get different bands.

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