Question

How are fluorescent cycle sequencing products separated so that the nucleotide sequence can be determined?

Answer 1

Determining the order of the nucleotides within a gene is known as DNA sequencing. The earliest DNA sequencing methods were time consuming, but a major breakthrough came in 1975 with the development of the process called Sanger sequencing.

Sanger sequencing is modeled after the natural process of DNA replication, and it uses dummy nucleotides to stop replication whenever a specific nucleotide is encountered. Because this truncated replication occurs over and over again, nucleic acids of varying lengths accumulate and can be used to determine the position of each nucleotide in the sequence.