Question

4) Please explain how a fluorescent tag can be used to visualize protein localization in a cell.

Answer 1

With the advancement in the science, we can examine the proteins through probes and these probes act as marker to see the function or location of the specific proteins. In biological research, scientists have been working on RFP and GFP tagged protein and this specific florescent agent act as reporter gene for the functional group of proteins. They functional or encoded protein move inside the cell then this reporter marker also move as it is combined with the protein and under specific light fluorescent or UV light we can easily localized the position of the protein (on the membrane or cytoplasm or nucleus). Confocal for best resolution can be used for localization and fluorescent microscope can also detect the signal for the same.