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After a restriction digestion of a TOL plasmid, why should the enzyme be inactivated immediately after...

After a restriction digestion of a TOL plasmid, why should the enzyme be inactivated immediately after the restriction experiment? How can the inactivation be achieved?

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Immediately after the restriction experiment the restriction enzymes should be inactivated immediately by treating with inactivation temperature (heat treatment) at which the particular used restriction enzyme inactivated . These RE should be immediately inactivated because RE enzymes have a activity time for proper restriction but if their activity remain activefor long time than they can cause overdigestion of plasmid and multiple fragments of TOL plasmid will obtain that is not the result we want.

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