Question

What was the purpose of performing flow cytometry with permeabilized and fixed

erythrocytes?

Answer 1

Fixation and permeabilized erythrocytes will allow us to determine the cell shape, surface proteins and internal molecules as close to it's natural state as possible in the process of preparing erythrocytes.

Fixation is the prevention of biological calls from decay due to autolysis or putrefaction.

By flow cytometry detection of erythrocytee(Red blood cell) bound anemia (Auto immune haemolytic anemiaa aplastic anemia and missing proteins like CD55 CD59 on the surface of erythrocytes, measuring haemoglobin, internal cell molecules) can be done.