Question

what is the purpose of including a blank paper disc on a kirby-Bauer agar plate ?

what is the purpose of including a blank paper disc on a kirby-Bauer agar plate ?

Homework Answers

Answer #1
  • In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and antibiotics (white disks, shown) are added to the plate.
  • After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
  • A blank disc is act as the control. The purpose of the control is two-fold. First, it helps to show what normal growth of bacteria looks like. Second, it helps to show that the antibiotics, and not the paper, are affecting the bacteria.
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