Question

what is the purpose of including a blank paper disc on a kirby-Bauer agar plate ?

what is the purpose of including a blank paper disc on a kirby-Bauer agar plate ?

Homework Answers

Answer #1
  • In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and antibiotics (white disks, shown) are added to the plate.
  • After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
  • A blank disc is act as the control. The purpose of the control is two-fold. First, it helps to show what normal growth of bacteria looks like. Second, it helps to show that the antibiotics, and not the paper, are affecting the bacteria.
Know the answer?
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Not the answer you're looking for?
Ask your own homework help question
Similar Questions
Imagine that you have set up a Kirby Bauer experiment on Mueller Hinton agar (non-selective agar)...
Imagine that you have set up a Kirby Bauer experiment on Mueller Hinton agar (non-selective agar) in order to test if Escherichia coli is susceptible or resistant to Drug X. After 24 hours, you notice a zone of inhibition (clear zone) around your antibiotic disc and determine that E. coli is susceptible to Drug X. You then take a cotton swab and subculture a sample from this zone of inhibition (where no growth was apparent) onto a new agar plate...
Which of the following could be used as a negative control in Kirby-Bauer disc diffusion? Please...
Which of the following could be used as a negative control in Kirby-Bauer disc diffusion? Please explain A. A disc containing bleach. B. A disc containing glucose C. A disc containing streptomycin D. A disc containing deionized water
What would happen to the result if the Kirby-bauer test was run at room temperature (25...
What would happen to the result if the Kirby-bauer test was run at room temperature (25 degrees celsius), instead of 37 degrees?
What is the purpose of kanamycin in the agar plates?
What is the purpose of kanamycin in the agar plates?
What is the purpose of running separate test in motality test agar
What is the purpose of running separate test in motality test agar
what is the purpose for a TSA plate?
what is the purpose for a TSA plate?
Who issues commercial paper and for what purpose?
Who issues commercial paper and for what purpose?
What is the purpose of including baffles on the shell side of the heat exchanger? Consider...
What is the purpose of including baffles on the shell side of the heat exchanger? Consider the impact on velocity, Reynolds number, and turbulence, as well as prevention of flow channeling.
Research the current history of "ban the box" including the purpose of these laws; what states...
Research the current history of "ban the box" including the purpose of these laws; what states they currently are active, and what are the pros/cons of both sides of the issue.
QUESTION 1.What is the purpose of a research paper and why cant I use first? 2.How...
QUESTION 1.What is the purpose of a research paper and why cant I use first? 2.How do i write a research paper in thrid person? i know that i cant use i or me but what should i say instead?
ADVERTISEMENT
Need Online Homework Help?

Get Answers For Free
Most questions answered within 1 hours.

Ask a Question
ADVERTISEMENT