Question

What would happen to the result if the Kirby-bauer test was run at room temperature (25 degrees celsius), instead of 37 degrees?

Answer 1

Answer:

Kirby- Bauer test is basically used to test and evaluate antibiotic sensitivity. It is also called as disk diffusion test. In this mainly used agar medium and 30% poloxamer hydrogels at 25°C, because at 37°C zone of inhibition is not clear, non- measurable and accurate results were not recorded. As poloxamer hydrogels results in gel formation and it is temperature dependent (i.e., liquid below 15°C) and becomes readily reversible when the petri-dishes are placed at the temperature at 25°C during recording measurement shows clear zone of inhibition at 25°C and produces accurate results.

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