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Hi, In organic chemistry Column Chromatography lab experiment there was a question saying What may happen...

Hi, In organic chemistry Column Chromatography lab experiment there was a question saying

What may happen if the level of the developing solvent is allowed to fall below the sand layer?

What would be the correct answer for it?

thanks

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Answer #1

The column Chromatographic Technique is used to Separate Organic compunds, and this is achieved by the "ADSORPTION" of Organic compunds on the surface of Silica

Sand Layer plays an important role in Column Chromatography as they protect column of Silica from any kind damage,due to which uniform bands are formed and effective separation will going to take place

If Developing solvent is allowed to fall below the sand layer the silica column will become DRY and due to which the separting band will merge and the rate of Elution will fall down and separtion will be affected.

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