Question

Column Chromatography Theory and Background. I have started but would like to see an example of what can else can be used.

Some key points in my background need to include. Describe Column chromatography and its applications Choosing solvents for column chromatography sample and column size packing column two methods, adding sample, eluting column, and monitoring column

Column chromatography is used to purify individual chemical compounds from mixtures. Generally, it is used in a glass tube from 5 mm to 50 mm and a height of 5 cm to 1 m. Two methods that can be used are the dry method which the column is filled with a dry stationary phase powder and followed by the mobile phase, then flushed through the column until wet. And the wet method, in which a slurry is prepared with the stationary phase powder and then poured into the column. A solution of organic material is carefully added to the top of the stationary phase. The layer is then topped with small amount of sand, cotton, or glass wool to protect the shape.

The solvent that is generally chosen for column chromatography depends on the polarity of what is being separated. Hexane or cyclohexane is often used for non-polar compounds.   Ethyl acetate is often used for polar compounds. Similar to TLC, the solvents can be mixed in a ratio to separate the chemicals with better results.

Answer 1

The components of the mixture to be separated have different affinities for stationary and mobile phase. As the solvent system moves down the column, the individual components of the mixture to be separated move through the column at different speeds. They come out of the column one at a time. The eluent is collected in a series of fractions. These fractions are analyzed with TLC to check in which fractions, each component is present. Better separation is obtained when we increase the flow rate of eluent.