Question

Why would you add EDTA in a buffer solution if you are performing an experiment where LDH is extracted from a liver. Why is it beneficial to us an EDTA buffer before homogenizing?

Answer 1

EDTA is used for protein purifcation. Here protein is LDH which is extracted from liver.

We will perform a two step purification of LDH extracted from liver. Lactic Acid Dehydrogenase in an important enzyme in the anaerobic metabolism of glucose for the generation of ATP. The activity of this enzyme is primarily responsible for explosive anaerobic athletic activity. The reaction that the enzyme catalyzes is the interconversion of pyruvate and lactate. Under anaerobic conditions, pyruvate is converted to lactate, with the concomitant conversion of NADH to NAD⁺. The regeneration of the NAD⁺ permits continued metabolic flux down the glycolytic pathway. This flow continues until the energy demands cease or until the cell and blood levels of lactate become intolerably high.

This is why is it beneficial to us an EDTA buffer before homogenizing