Question

you have a stock solution of 0.7% and a buffer as a diluting medium. In a testtube, you have to add 2 mL of a protein solution. you have to make 5 different dilutions that range between 0.02% and 0.30%. The final volume of the solution is 7 mL. Which formula would you use to calcuate the volumes of detergent and amount of buffer needed to make this dilution series?

Answer 1

As you have to make 5 different dilutions that range between 0.02% and 0.30%. You can make these dilutions by serial dilution for that you have to determine the value of three variables thai is df (dilution factor which is in this series is 100), v2 (for each dilution v2 is the volume which you want to make) and v1 (v1 is the more concentrated solution that you mix with solvent to make the more dilute solution). When you know df and v_2, you can calculate the value of v₁ using formula as fllows-

v1+v2/v1=df

By this formula you can calcultate the volumes of detergent and amount of buffer needed to make this dilution series