Question

You have 2 enzymes, A and B that can act of substrate S. Enzyme A has...

You have 2 enzymes, A and B that can act of substrate S. Enzyme A has a higher Km than enzyme B. Which enzymes will be active under the following conditions:
a) high [S]
b) low [S]

Homework Answers

Answer #1

Michaelis–Menten equation is used typically in enzymatic reactions.

V = Vmax*[S] / (Km + [S])

Where

Vmax = max rate velocity

[S] = substrate concentration

Km = Michaelis–Menten constant

V = reaction rate

a)

if [S] high --> V = Vmax*[S] / (Km + [S]) --->

for A (high KM) -> V = Vmax*[S] / (Km + [S])

for B (low Km) --> V = Vmax*[S] / (Km + [S]) = Vmax*[S] / ([S]) = Vmax

therefore, B

b)

low S

for A (high KM) -> V = Vmax*[S] / (Km)

for B (low Km) --> V = Vmax*[S] / (Km + [S]) = Vmax [S]/[S] = Vmax

then A

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