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What is the order in which the following compounds would be eluted from a HPLC column...

What is the order in which the following compounds would be eluted from a HPLC column containing a normal-phase packing? Briefly justify your reasoning.
phenol, benzoic acid, aniline, chlorobenzene

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Answer #1

In normal phase, the stationary phase is polar and mobile is non-polar.

So, the molecule which is the most non-polar will elute first and the most polar will elute tha last.

Among the given four compounds aniline id the most polar, then benzoic acid, phenol and the least polar ic Chlorobenzene.

The order of eleution wlii be

Chlorobenzene > Phenol > Benzoic acid > Aniline

Polar compound will have strong interaction with polar stationary phase than the non-polar compounds.

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