Question

(a) In what order would the amino acids Arg, His, and Leu be eluted from a...

(a) In what order would the amino acids Arg, His, and Leu be eluted from a carboxymethyl column at pH 6?

(b) In what order would Glu, Lys, and Val be eluted from a diethylaminoethyl column at pH 8?

Can anyone show me step by step how to solve this? I don't understand how to find the charges of the amino acids and how pH affects it?

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