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Which compound and why would you expect to have a shorter retention time on a reverse-phase...

Which compound and why would you expect to have a shorter retention time on a reverse-phase HPLC column with a water/acetonitrile mixture adjusted to pH3 with acetic acid — benzylalcohol or benzoic acid.

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Answer #1

In reverse phase HPLC column, the stationary phase is nonpolar. Between, benzyl alcohol and benzoic acid, benzyl alcohol is less polar as compared to benzoic acid. Less polar benzyl alcohol is thus going to have more attractive intermolecular interaction with the stationary phase as compared to benzoic acid. Hence, benzoic acid will elute first when eluted with water/acetonitrile mixture adjusted to pH 3 with acetic acid . Therefore, benzoic acid is expected have a shorter retention time.

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