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Explain why you chose the answer. What is the advantage of an open tubular column relative...

Explain why you chose the answer.

What is the advantage of an open tubular column relative to a packed column in gas chromatography? Why is longitudinal diffusion a more serious problem in gas chromatography than in liquid chromatography? Why does the efficiency of liquid chromatography increase as the stationary phase particle size is reduced?

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Answer #1

Compared to packed columns, open tubular columns can provide:

1. Higher Resolution
2. Shorter analysis time
3. Increased sensitivity
4. Lower sample capacity

Compared to packed columns, open tubular columns allow:

1. Increased linear flow rate or a longer column or both
2. Decreased plate height, which means higher resolution

Diffusion coefficients of gases are 10^4 times greater than those of liquids. Therefore, longitudinal diffusion occurs much faster in gas chromatography than in liquid chromatography.

The resolution equation comprises three terms: selectivity, retention capacity, and efficiency. Each of these terms is affected by the specific components of an analytical method. A column’s particle size, in particular, affects the efficiency term of the resolution equation.

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