Question

2.) True of False? Justify.

(a) Fluorescence spectroscopy is a more selective method than UV spectroscopy.

(b) Energy of excitation light is higher than energy of fluorescence light for a given molecule

(c) In general, fluorometry sensitivity is lower than in UV/Vis spectrometry.

(d) At low concentration, intensity of fluorescing light is directly proportional to analyte concentration.

Answer 1

1) False, Uv spectroscopy is more selective and general method

2) emitted fluorescence light has a longer wavelength and lower energy than the absorbed light

3)The sensitivity of fluorescence detection is approximately 1,000 times greater than absorption spectrophotometric methods. Only molecules that fluoresce are detected by this method, resulting in greater specificity compared with UV/Vis absorption

4) concentration of the analyte is directly proportional with the intensity so TRUE