The turning off or on a gene are the events which occurs in operons. There are negatively and positively controlled operons.
An inducer is needed to on the structural genes. It happens when there is it's requirement. Like in lac operon, when lactose is present it acts as inducer and binds to the repressor, so that RNA polymerase don't get any interruption in transcription. The translation forms products required for the lactose nutrition.
Similarly when lactose is absent, the repressor binds to the operator region of the lac operon, so the structural genes are switched off and no products (enzymes) are produced.
Polymerase chain reaction amplifies the DNA amount by producing many copies. It has probably no role in the gene regulation.
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