1. an isolated colony is developed from a single organism to
maintain a pure and genetically unmutated sample of bacteria and
also bacteria will die due to overpopulation and starvation if they
are not replaced in another media which contains new nutrients for
it to grow.
2. Biological control, is the use of living organisms to control
pests. A natural enemy such as a parasite, predator, or disease
organism is introduced into the environment of a pest and is
encouraged to multiply and become more effective in reducing the
number of pest organisms.
(the question seems vague)
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