Question

1) What is meant by the "bottleneck" to microbial ecology?

2) Why is it important to ensure all colonies on each streak plate have the same morphology before proceeding with molecular analysis ?

3) You are studying a microorganism from a hot spring that uses organic carbon as an energy and carbon source. The microorganism is a facultative anaerobe and can use nitrate as a terminal electron acceptor. You lab partner inadvertently mixes your isolate with a number of other microorganisms in the lab. To continue your research, you need to re-isolate the hot spring microbe. Answer the following in regard to the enrichment medium and incubation: → What components are absolutely essential? What will be your incubation conditions? → Generate a flow chart outlining the steps you will need to take to recover your original organism. → How would you change your enrichment procedure to include Archaea?

Answer 1