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Would you expect the concentration of hydrogen ions in the matrix of rotenone-poisoned mitochondria to increase,...

Would you expect the concentration of hydrogen ions in the matrix of rotenone-poisoned mitochondria to increase, decrease or remain about the same relative to mitochondria that are not poisoned with rotenone?


increase
decrease
remain about the same
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Answer #1
  • The mechanism of oxidative phosphorylation occurs in mitochondria of live cells.
  • Electrons are transported across various components of electron transport chain (ETC), resulting in series of oxidation-reduction reactions within the membrane bound complexes in mitochondria (complex I, II, III, IV).
  • NADH and FADH2 are electron donors, their oxidation creates high concentration energy from electrons.
  • This energy is used to pump proton from matrix to inter membrane space. Flow of protons creates and electrochemical gradient, which is used to generate ATP.
  • Rotenone inhibits electron flow at complex I.
  • This will prevent energy creation to transfer protons from matrix to mitochondrial membrane.
  • Thus, concentration of protons or hydrogen ions will increase in matrix.

Answer: increase

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