Question:Would you expect the concentration of hydrogen ions in the
matrix of rotenone-poisoned mitochondria to increase,...
Question
Would you expect the concentration of hydrogen ions in the
matrix of rotenone-poisoned mitochondria to increase,...
Would you expect the concentration of hydrogen ions in the
matrix of rotenone-poisoned mitochondria to increase, decrease or
remain about the same relative to mitochondria that are not
poisoned with rotenone?
The mechanism of oxidative phosphorylation occurs in
mitochondria of live cells.
Electrons are transported across various components of electron
transport chain (ETC), resulting in series of oxidation-reduction
reactions within the membrane bound complexes in mitochondria
(complex I, II, III, IV).
NADH and FADH2 are electron donors, their oxidation creates
high concentration energy from electrons.
This energy is used to pump proton from matrix to inter
membrane space. Flow of protons creates and electrochemical
gradient, which is used to generate ATP.
Rotenone inhibits electron flow at complex I.
This will prevent energy creation to transfer protons from
matrix to mitochondrial membrane.
Thus, concentration of protons or hydrogen ions will increase
in matrix.