Question

3. A receptor protein (RPX) has been shown to be involved in mediating transcription of gene X. RPX has a binding region for metabolite (M). In the absence of metabolite, acetylase p300 is recruited to form a complex involved in chromatin remodelling. In the presence of metabolite, deacetylase HDAC1 is recruited to form a complex involved in chromatin remodelling.

A) In the absence of metabolite, how would you characterize the role (one word) of RPX regarding transcription? Briefly explain your reasoning.

B) What is the effect of the metabolite on the transcription of gene X? Explain briefly your reasoning.

C) What amino acid residue would most likely be a substrate for p300?

Answer 1

Answer 1.

In the absence of metabolites, RPX would not be bind with metabolites(M). Hence, enzyme acetylates ( Acetylase (p300)

Answer

a) In the absence of metabolite RPX would not be bind with metabolite. Hence enzyme that acetylate( Acetylasep300)Will be recruited and the process of chromatin remodeling will be initiated. This will activate the X gene expression.

b) In the presence of metabolites deacetylase, HDAC1 have shown the opposite effect of removing the acetyl group and promoting the transcriptional repression of gene X.

c) Specific lysine residue would most likely to be present in the gene X and substrate for the enzyme Acetylase p300. Lysine is the amino acid that undergoes the process of acetylation for the activation of gene expression