Ans. A flouroscent microscope is used to study flouroscence-labelled cells. In this, light is passed through excitation filter that selects the wavelength tgat excites the specimen. The wavelength is reflected towards specimen using dichroic mirror. The excited specimen emits light which passes through dichroic mirror and a barrier filter, which select light of wavelength emitted by flouroscent specimen.
Advantages - It provides insight into the dynamic changes in cells. And is more relevant and reliable than fixed cell micrography. And using different fluorophores in the same sample can also allow the simultaneous observation of several molecules at the same time.
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