Question

1. What are the advantages of Y-chromosome STR profiling? Select the two correct answers.

Less genetic variability exists on the Y chromosome than on autosomal chromosomes.

Y chromosome STR profiling is possible for more than 200 loci.

Gender separation of mixed tissue sample is easily achieved.

Y chromosome STR profiling is more sensitive than standard STR profiling.

2. What are the limitations of this procedure?

Y chromosome doesn't undergo recombination, thus it is difficult to differentiate DNA from close male relatives.

STRs are longer on the Y chromosomes, which leads to less efficient amplification of DNA.

Y chromosome DNA is hypermetilated, thus it is more difficult to amplify.

Sperm cells do not always contain Y chromosome, therefore sometimes semen cannot be matched with the suspect.

3. Choose the correct description of capillary electrophoresis.

Capillary electrophoresis uses thin glass tubes containing agarose gel to which amplified DNA is loaded onto the top of the tube.

Capillary electrophoresis uses slab polyacrylamide gel to which amplified DNA is loaded onto wells.

Capillary electrophoresis uses thin glass tubes containing polyacrylamide gel to which amplified DNA is loaded onto the top of the tube.

Capillary electrophoresis uses slab agarose gel to which amplified DNA is loaded onto wells.

What are the applications of this technique? Select the three correct answers.

scientific research

pathogen detection

paternity tests

crime investigation

gene therapy

Answer 1

1. Two correct options are:

Y chromosome STR profiling is possible for more than 200 loci.

Gender separation of mixed tissue sample is easily achieved.

2. Two correct options are:

Y chromosome doesn't undergo recombination, thus it is difficult to differentiate DNA from close male relatives.

Sperm cells do not always contain Y chromosome, therefore sometimes semen cannot be matched with the suspect.

3. Correct option is:

Capillary electrophoresis uses thin glass tubes containing polyacrylamide gel to which amplified DNA is loaded onto the top of the tube.

4. Three correct options are

scientific research

paternity tests

crime investigation