Question

What is the advantages of using fluorescence rather than colorimetric methods for immunohistochemistry/immunocytochemistry and in situ hybridisation? (4)

Answer 1

Florescence serves as a better method than colorimetry for immunohistochemistry, in situ hybridization because-

1. Offers studying multiple targets- More colors can be used for studying various proteins, molecules, receptors etc.

2. Colocalization of the target is better- When the targets are co-localized, florescence helps in better visualization. It helps them in identifying separately.

3. Dynamic range is higher- It helps to visualize rare targets as well as abundant targets simultaneously. They can be done on one slide.

4. Easy to perform and high sensitivity- It is less time consuming and can give results in fewer steps.

However, there are some limitations as well. It is susceptible to photo bleaching.