Question

5. You are studying a new disease in frogs and want to follow Koch’s postulates. What is your second step?

a. Determine the shape of the bacterial cells.

b. Inject the bacteria into a healthy frog.

c. Isolate the bacterium from a sick frog.

d. Show that the bacterium is not present in healthy frogs.

e. Grow a pure culture of the bacterium outside the frog.

Answer 1

Ans e. Grow a pure culture of the bacterium outside the frog.

The first step would be option c , isolate the bacterium from a sick frog .

Second step- pure culture

Third step- inject the bacterium into a healthy frog

Fourth step- disease reproduced in the healthy frog and the bacterium reisolated and cultured.

Koch's postulates are-

1. The microorganism must be present abundantly in every sick organism.

2. Should be able to isolate the bacteria and culture it .

3. When innoculated into a healthy host , must reproduce the disease.

4. Should be able to reisolate the bacteria from the lesions of the experimental host organism.

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