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Explain what you would do to make a 10.0 mL of a 10 μM solution of...

Explain what you would do to make a 10.0 mL of a 10 μM solution of caffeine in water, starting directly from caffeine powder. The molecular weight of caffeine is 456 g/mol. Be detailed in the order in which you would add chemicals and their amounts. Is there something wrong with this? Without being very wasteful, by what other means might you make up this solution?

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Answer #1

Molarity = mass/molecular weight * 1000/ volume in ml.

Molarity = 10*10-6 M

Mass need to find out

Molecuale weight of caffeine = 456g/mol

Volume = 10ml

10*10-6 = mass / 456 * 1000 /10

Mass = 10*10-6 *10/1000

Mass= 45.6 microgram.

Since this weight is very low and cannot be measured.

So we need to prepare a stock of 10mM. For 10mM we need 456mg of caffeine.

Dilute the 10mM solution three times 10 fold serially as shown in the figure.

Please write to me if you need more help.

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