Question

Please list the reagents and apparatus you need in order to perform a restriction reaction in the laboratory.

Answer 1

Reagents -

1. Restriction enzyme - enzyme which will cut the DNA at a specific site called as restriction site

2. DNA which is to be digested/cut

3. Buffer - which will help to maintain an optimum pH for the working of restriction enzyme

4. Sterile water - to make up a specific final volume

Apparatus -

1. PCR tube - in which all the reagents will be added

2. Microtips and micropipettes - for transferring small volume of the reagents

3. PCR tube holder to keep the PCR tubes

4. Incubator to incubate the reaction mixture at 37℃

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