Question

List the steps below in the correct order to describe how to perform a Southern blot.

	Perform a blot onto nitrocellulose paper
	Restriction digestion of a sample
	Perform gel electrophoresis on the sample.
	Apply probe to sample
	Expose sample to a radiosensitive film

Answer 1

Restriction digestion of a Sample perform gel electrophoresisPerform a blot onto nitrocellulose paper Apply probe to sample Expose sample to the radioactive film

We can define the southern blotting as a method to detect any particular DNA sequence of a given sample. The method of blotting comprises the study of fragmented DNA sequences on the filter membrane which is transferred from the gel electrophoresis. Then the probe to expose the sample to a radioactive film. That is how the method of southern blotting done the DNA study