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You are studying the Hox genes in a new animal model system. How would you determine...

You are studying the Hox genes in a new animal model system. How would you determine where the genes are expressed? Briefly explain ONE method you could use (points will be deducted if you describe more than one method).

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Solution: Hox genes or homeobox trnscription factors are important specifiers in the development of limbs in tetrapods. Different regions of limb, such as stylopod (proximal), zeugopod(middle) and autopod(distal) regions are specified by different hox genes. Hox 9 and Hox 10 genes specifies stylopod. Hox 11 specify the development of zeugopod, and Hox 12 and 13 specifies autopod development. This is determined by mutational studies. When Hox 10 gene is mutated the femur development in hind limb ceases. If the Hox 11 is mutated, femur in hind limb and humerus in fore limb develops but further development of limb structures would not occur.

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