Question

You are screening newborns to see if they have the progerin gene mutation or not.  Describe how you would use FISH as a diagnostic toolfor this disease. What technique could you use to see if the progerin protein or normal Lamin A protein was being expressed.  For both, explain the technique and how you would determine which protein the patient expressed (the normal or progerin). Everything you need to know about the progerin gene and protein is in the Human Connections section.

Please understand that GFP-fusion is a research technique and cannot be used as a patient screening technique. The GFP-fusion is done in the lab (molecular cloning) and done with cells grown in the lab. No humans have GFP-fusion proteins expressed in them (or else you would be a transgenic person).

Answer 1

Answer : as per FISH technique the DNA probe is labelled and indirectly with a haotenor directly labelled via incorporation of a fluorophore.

That's why this technique is called flurocence in situ hybridization to detect the chromosomal abnormality.

A cytogenic equivalent that exploits the ability of single stranded DNA to anneal to complementary DNA. In FISH the target is nuclear DNA of interphase or metaphase chromosomes affixed with a microscopic slide.

The labelled probe and the target DNA are determined to yield single stranded DNA.

They are combining which allows the annealing of complementary DNA sequence.

This is how FISH works and detects the disease.

An ELISA technique could be designed to detect the correct protein. But for this antibibosies should also be designed with the respective enzymes.