Question

25. In recombinant DNA, a DNA probe is used to: transfer DNA into a cell synthesise...

25. In recombinant DNA, a DNA probe is used to:

  • transfer DNA into a cell
  • synthesise DNA for cloning
  • separate pieces of DNA
  • find a specific nucleotide sequence

Homework Answers

Answer #1

(25) First let us know what is DNA probe.

  • DNA probes are generally small, single stranded fragments of DNA which is complementary to the target sequence or the target DNA sequence.
  • The probes can be generated as inserts in the plasmids by recombinant DNA techniques.
  • DNA probes generally employs a method called nucleic acid hybridization to rapidly detect complementary sequence in the given sample or test sample.
  • Thus DNA probes are used to detect the presence of specific nucleotide sequence by nucleic acid hybridization in the target sequence.
  • These DNA probes are usually labeled (for eg. with radio isotopes) which enables their detection.

So the answer is:

In Recombinant DNA, the DNA probe is used to

* find a specific nucleotide sequence

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