Question

Which of the following involves the insertion of a chemically synthesized DNA containing an insertion/deletion of a nucleotide in the sequence to create specific genetic change?

A. Combinatorial biosynthesis
B. Site-directed mutagenesis
C. Metagenomics
D. Protoplast fusion

Which of the following is true about restriction enzymes?

A. Recognizes non-specific sites of DNA

B. Used to promote viral infections

C. Can cut a DNA fragment to create “sticky” ends that are utilized for generating recombinant DNA

D. A and C

Answer 1

1- insertion of chemical synthesize DNA or primer to induce mutation in the original DNA sequence is called as site-directed mutagenesis.

SO answer is option B. Site-directed mutagenesis

2- Restriction ENzymes cleaves the specific DNA sequence and cleaves the DNA at that sequence. Upon digestion, they can produce sticky end or blunt end which can be ligated by using ligase.

SO answer is option C- C. Can cut a DNA fragment to create “sticky” ends that are utilized for generating recombinant DNA