Question

How is dCas9 different from cas9? They bind to a specific DNA sequence within a cell....

How is dCas9 different from cas9?
They bind to a specific DNA sequence within a cell.
Cas9 is used for studying gene function while dCas9 is used for gene editing.
They do not have a nuclear function so don’t cut target DNA.
They use a guide RNA to locate a specific DNA sequence within a cell.
Cas9 is found in prokaryotes but dCas9 is found in eukaryotes.
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Cas9 is found in prokaryotes and dCas 9 is found in eukarotes


Cas9 Endonuclease Dead, also known as dead Cas9 or dCas9, it can be produce in the mutation in cas9. In cas 9 endonuclease activity is removed with the help of point mutaton in its domain. Its is unmated form dCas9 is used in CRISPR systems which is target specific genes , with PAM sequences nucleotides complementary to gRNA which allow cas9 to bind . in cas9 endonuclease is not present but it has 2 domains of it – RuvC and HNH domain

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