Question

using variable wavelength slider how would you use the relationship on transmission and absorvance to best measure the concentration of a solution

Answer 1

The concentration of the solution is measured with the help of beer and lambert's law. This depends on the absorbance of the solution and the path length of the cuvette along with the molar extensive coefficient. Thus the absorbance of the liquid sample which has been provided is based on the light which bombards on the cuvette and number of molecules present in the solution which is actually the concentration of the solution. Thus, the initial light is named as the incident light while the light which has been absorbed by the molecules to move from the ground state to the excited state is released or transmitted while coming back to ground state and this is known as the transmitted light. Thus the ration of the incident light amount to that of the transmitted light is known as the absorbance which is given by the spectrophotometer.

In this technique to find out the actual concentration of the solution variable wavelength of the lights are used and that are to find out the wavelength max that is which wavelength can be absorbed more by the molecule and allow the system to give the highest peak which will be distinct from the other peaks and thus it will be considered as this wavelength will be used to find out the concentration of a particular molecule solution.

Therefore, the variable wavelength is used for analyzing the transmission and absorbance to detect the concentration of the solution.