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If a piece of linear DNA is cit at eight different sites by a restriction enzyme...

If a piece of linear DNA is cit at eight different sites by a restriction enzyme and then put into a gel for electrophoresis, how many bands will be present in the gel?
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Answer #1
  • The endonuclease is the restriction enzymes that cut the DNA at the specific sites so that DNA is cut to produce sticky ends. Let us consider the number of restriction sites be N then the number of fragments produced from cutting linear DNA will be N+1.
  • Each fragment corresponds to be each band in gel electrophoresis.
  • So given number of the restriction sites is 8
  • So the number of treatments = number of bands = 9
  • So that the number of bands present on the gel electrophoresis is 9 bands, but they are different in size which helps in its separation.
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