Question

From the list below please fill in the blanks

-blunt

-chromatography

-clone

-complementary

-dideoxy sequencing

-DNA polymerase

-DNA replication

-electroporation

-gel electrophoresis

identical

karyotype

ligase

next-generation sequencing

plasmidre

combinant

staggered

transcription

translation

Any two DNA molecules cut with the same restriction enzyme will generate the same cohesive ends.  These cohesive ends have single-stranded overhangs that will be ______to each other.  Incubating such fragments together with the enzyme ______can result in the formation of ______ molecules, which have parts that originate from different organisms.To exchange DNA molecules between cells, a double-stranded circular DNA molecule is often used as a vector. This type of molecule, known as a______, can replicate independently in its host cell, and can be used to generate many copies of the desired DNA sequence.  In general, to produce of many copies of a DNA fragment is to that fragment.

Answer 1

Any two DNA molecules cut with the same restriction enzyme will generate the same cohesive ends.  These cohesive ends have single-stranded overhangs that will be identical to each other.  Incubating such fragments together with the enzyme ligase can result in the formation of recombinant molecules, which have parts that originate from different organisms.To exchange DNA molecules between cells, a double-stranded circular DNA molecule is often used as a vector. This type of molecule, known as a plasmid, can replicate independently in its host cell, and can be used to generate many copies of the desired DNA sequence.  In general, to produce of many copies of a DNA fragment is to that fragment.