Question

Construct the physical map of a DNA molecule from which fragments of different sizes were generated after digestion with the following restriction enzymes:
-ApaI: generates a 5.8 kb fragment
-BamHI: generates two fragments of 2.5 and 3.3 kb
-PstI: generates a 5.8 kb snippet
-ApaI / BamHI: generate three fragments of 1.3, 2.0 and 2.5 kb
-ApaI / PstI: generate two fragments of 1.0 and 4.8 kb
-BamHI / PstI: generate three fragments of 0.3, 2.5 and 3.0 kb
-ApaI / BamHI / PstI: generate four fragments of 0.3, 1.0, 2.0 and 2.5 kb
a) Is the molecule circular or linear?
b) How many cutting sites do you have with each enzyme?
c) How many fragments, what size and what conformation would the products generated if partial digestion with the ApaI enzyme occur?

Answer 1

Please look in the image for solution:

The number of sites is equal to the number of fragments in the plasmid.

The molecule is circular because map is found only in circular form from the given conditions.

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