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If you do a PCR reaction with DNA from a person who is heterozygous for a...

If you do a PCR reaction with DNA from a person who is heterozygous for a SNP in the gene you are amplifying and you have a restriction enzyme that will recognize and cut only the one of the two allleles, how many bands will you see if you run the restriction digest out on an agarose gel?

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Answer #1

Given that person is heterozygous for the trait. It means he has one normal allele and one mutated allele. Given that one allele is recognized by the restrictions enzyme and give two bands on the gel. So the other allele is not recognized y the enzyme and give only one band.

Since the person is heterozygous for the SNP, one of the alleles will give two bands and another allele will give one band. So, total number of bands will be 3.

So, there will be 3 bands on the gel.

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