The line of identity in a gel immunoassay
Indicates that the antibody and antigen are specific for one another |
||
does not occur in double diffusion gel precipitation |
||
Occurs at the equivalence site of antibody/antigen reaction |
||
Both 1 and 3 |
Ans.. Both 1 and 3
when the same antigen is present in both wells each line of precipitation becomes a barrier for the antigen and antibody involved, preventing them from diffusing past the precipitin line. This shortens the precipitin line on that side of the well.
Complete fusion of precipitin lines with no spurs is called a line of identity, which indicates that the antigen in each well reacts with all the antibody capable of reacting with antigen in the other well.
double immunodiffusion test have following objectives:
Get Answers For Free
Most questions answered within 1 hours.