Question

In a flow cytometer, the amount of light scattered by a cell at a 90 degree...

In a flow cytometer, the amount of light scattered by a cell at a 90 degree angle (side scatter) is detected by a_____________.

1.

tungsten lamp
2.

laser
3.

photodiode
4.

photomultiplier tube
5.

phototube
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Answer #1

In a flow cytometer, the amount of light scattered by a cell at a 90 degree angle (side scatter) is detected by a :-

4. Photomultiplier tube

A photomultiplier tube (PMT) is used to measure the side scatter because it is more sensitive optical detector. PMT is required since light signals from side scatter are weak relative to forward scatter. Side scatter provides information about internal complexity (granularity) of cell.

A photodiode is used in measurement of forward scatter which converts light signal into electrical signal.

Since PMT is more sensitive than phototube so PMT is used most commonly.

Laser & tungsten lamp are the light sources not the detector.

So the correct answer is (4) Photomultiplier tube.

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