Which of the following cells should scatter the most light in the side angle?
1. |
red blood cell |
|
2. |
monocyte |
|
3. |
lymphocyte |
|
4. |
neutrophil |
The side scatter of light occurs due to the presence of a large number of tiny particles in the cell. Thus, the side scatter gives information about the complexity of the internal environment of the cell. The granular the cytoplasm, the more side scatter there is.
Red blood cells are simple cells without a nucleus. They merely act as oxygen-carrying agents and do not have granularity.
Monocytes and Lymphocytes are agranular cells and have large nuclei.
Neutrophils are granulocytes (other granulocytes are Eosinophils and Basophils). They have a dense cytoplasm with a large number of granules.
Thus, of the four cell types, only Neutrophils are granular and hence have high internal complexity. Therefore, they will be the ones to give the largest side scatter.
Hence, the correct option is Neutrophil.
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